Storage buffer

Storage Buffer Preparation for R5(2)-mCh-FL-BST

final concentration (with pH 7.1):

storage buffer recipe
Amount	Chemical
30 mM	KCl
2.5 mM	TRIS-HCl
0.025 mM	EDTA
1 mM	DTT
0.1%	Triton X-100

procedure for 250 mL:

1. weigh out the following powders: KCl, TRIS, EDTA, DTT

measured mass
	Desired Amount
KCL	559.14 mg
TRIS	75.71 mg
EDTA	1.83 mg
DTT	38.56 mg

1. add all chemicals directly to a 250 mL pyrex container
2. add 250 mL H₂O to container
3. add 250 μL Triton X-100
4. adjust pH to 7.25

Calculations:

Quantity calculator
	g/mol	desired mM	desired mL	required mg
KCL	74.5513	30	250	559.13475
TRIS	121.14	2.5	250	75.7125
EDTA	292.24	0.025	250	1.8265
DTT	154.253	1	250	38.56325